Analysis Note

ControlPOSITIVE CONTROL: Cytotrophoblastic columns, the endothelial and fibroblastic stromal cells of villi, and the large decidualized cells of decidual membrane in placenta (Hurskainen et al., 1996). Macrophages in arteries (Nikkari et al., 1996), and fibroblast, endothelial, and tumor cells in breast carcinomas (Soini et al., 1994; Visscher et al., 1994). Conditioned serum-free medium from (dexamethasone-treated) human fibrosarcoma HT-10801 (Nikkari et al., 1996) or endothelial HUVEC4 (Schnaper et al., 1993) cells.

Application

Western Blotting (non-reducing; Nikkari et al., 1996) (Use Ab at 1µg/mL for 2hrs at RT)

Immunohistochemistry (Acetone-fixed frozen; Hurskainen, 1996): 1:100 for 30 minutes at room temperature

Immunoprecipitation: 2-5 µg/mg protein lysate (use protein G to precipitate immune complex).

Inhibition of MMP-9 Activity: 2-4µg/mL (Visscher, 1994)



Optimal working dilutions must be determined by end user.

Anti-MMP-9 Antibody, clone GE-213 is an antibody against MMP-9 for use in IP, WB & IH.

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

Disclaimer

Manufactured by Daiichi Fine Chemical Co., LtdUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified human gelatinase B.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1150

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Specificity

Under non-reducing conditions, it recognizes proteins of 92 kDa and 86 kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-9 (MMP-9; also known as 92 kDa and collagenase IV or gelatinase B) of human MMP-9. MAB13415 reacts with the complex (~200 kDa) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1; Nikkari et al., 1996). It shows no cross-reaction with pro and active forms of MMP-2 (Nikkari et al., 1996). Clone GE-213 also shows very low reactivity to mouse MMP-9 proteins by western blot, but at high enough antibody and antigen concentrations some detection is possible. Mouse immunhistochemistry is untested. Cellular Localization: Peripheral cytoplasmic.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.